These different elements are chosen and optimized by the skilled person depending on the host cell used. After hybridization, the excess probe is removed and the hybrid molecules formed are detected by the appropriate method measurement of the radioactivity, of the fluorescence or of the enzymatic activity linked to the probe. Such a vaccine can also be prepared in the form of a composition containing a vector according to the invention, placed under the control of regulatory elements allowing its expression in humans or animals. As regards the vaccine formulations, these may comprise appropriate immunity of the adjuvants which are known to those skilled in the art, such as aluminum hydroxide, a representative of the family of muramyl peptides as one of the peptide derivatives of N-acetyl-muramyl, a bacterial lysate, or alternatively incomplete Freund’s adjuvant. Thus, the protein arrays comprising a polypeptide sequence of the invention or an antibody of the invention are subject of the invention, as well as kits containing the necessary or. The vaccine compositions comprising nucleotide sequences will preferably comprise also the nucleotide sequences encoding a polypeptide or a fragment thereof corresponding to a protein, or a fragment thereof, of the cell envelope of Listeria.
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So it may be worth using unnatural amino acids such as D, or amino acid analogs, including forms sulphurous. The final wash is performed in 0. Said mutations may 317 to inactivation of the gene, or especially when they are located in the regulatory elements of said gene, an overexpression thereof. The invention relates to a method to identify specific nucleotide sequences from the genome of a bacterial strain of the genus Listenia, in particular a strain of L.
An alternative method, but less efficient automated ribotyping, presents a cost analysis, high mknton its use. In the case of a substitution, one mintno more acids amino consecutive or non-consecutive are replaced by the 20 amino acids “equivalent”. It is also noteworthy that the risk of listeriosis is variable depending on the strain of contaminating Listenia. Are also meant by microorganism associated in the present invention, any microorganism mp the nucleotide sequences or polypeptides according to the invention.
In particular, it is possible to identify nucleotide sequences according to the invention, facilitating secretion in such a prokaryotic or eukaryotic system.
Moreover, when the same plant is contaminated by two successive events it is important to show with certainty whether these are two independent mintkn or if the same strain is responsible for these two events.
Simplistically, one fixed representative peptides of different proteins of an organism on a support. These results are in mjnton with published data on the diversity of Listenia strains. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in the process transport and binding proteins.
CA2424952A1 – Listeria inocua, genome and applications – Google Patents
In particular, it is possible to study and determine the polymorphism regions between said strains. Thus, the protein arrays comprising a polypeptide sequence of the invention or an antibody of the invention are subject of the invention, as well as kits containing the necessary or. However, although some areas have not been cloned mintoh the bank, because of lethalities problems in Escherichia coli, these regions can easily be amplified and identified by the skilled person, using specific oligonucleotide sequences of ends of the different clones that form contigs.
In another aspect, mmpp, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in the adaptation to atypical conditions.
Cette infection est opportuniste et affecte miton les femmes enceintes, les This infection is opportunistic and primarily affects pregnant women.
In the mice, in which a weight dose of the vaccine composition 5 comparable to miinton dose used in humans mmo administered, the antibody reaction is tested by collecting serum followed by a study of the formation of a complex between the antibody present in the serum and the antigen of the vaccine composition, according to the usual techniques. The amplified nucleotide fragments may be used as reagents in hybridization reactions in order to highlight the presence l4, in a biological sample, of a sequence complementary to target nucleic acid to that of said amplified nucleotide fragments.
Strain Listeria monocytogenes serotype 4b is also identified in the present application by Mijton monocytogenes 4b and interchangeably. For polypeptide fragment according to the invention is meant a polypeptide comprising at least 5 amino acids, preferably 10, 15, 25, 50, and amino acids.
The polynucleotide or vector of the invention may also be suspended in a buffer solution or be associated with liposomes.
CAA1 – Listeria inocua, genome and applications – Google Patents
I1 is the etiologic agent of listeriosis, an infection related to food posing public health problems increasingly important, with significant economic impact for the food industry. The bifunctional reagents for this coupling are determined depending on the selected end to provide this coupling, and coupling techniques are well known to those skilled in the art.
I, in fact overlap respectively the genome of Listeria innocua and Listeria monocytogenes 4b. Des adaptateurs invitrogen Cat. Listeria monocytogenes is a facultative intracellular pathogen. Preferably, the probes or primers of the invention are immobilized on a support, covalently or noncovalently.
Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of this cell envelope or Listeria innocua or surface monocytogenes 4b or to a fragment thereof.
II must be stably maintained in the host cell and may optionally possess particular signals specifying the secretion of the translated protein. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria monocytogenes 4b imiocua or or a fragment thereof involved in the translation process. The present invention provides all the naturally active sequences in L.
Representative fragments of the invention can be obtained for example, by specific amplification such as PCR, or after digestion with appropriate restriction enzymes of nucleotide sequences according to the invention, this method is particularly described in Sambrook et al. Is intended to denote “best alignment” or “optimal alignment”, the alignment for which the percentage identity determined as below 5 is the highest.
All of these observations indicates that the genes identified as variables between L. Said hybrid nucleotide sequences coding for a hybrid polypeptide as well as the hybrid polypeptides according 3071 the invention characterized in that they are recombinant polypeptides obtained by the expression of said mintob nucleotide sequences 30177 also part of the invention.